OLIGONUCLEOTIDE FOR DETECTING THERMOSTABLE TOXICOGENIC PATHOGENIC ESCHERICHIA COLI AND DETECTION USING THE SAME OLIGONUCLEOTIDE

摘要

PURPOSE:To detect pathogenic Escherichia coli simply, rapidly and in high sensitivity by applying gene amplification method using an oligonucleotide complementary with a nucleotide sequence to code thermostable toxin-gene (ST gene) of pathogenic Escherichia coli as a primer. CONSTITUTION:An oligonucleotide to selectively detect pathogenic Escherichia coli having thermostable toxin (ST)-forming function existing in a test specimen or a nucleotide targeting a nucleotide sequence to code thermostable toxin-gene (ST gene) of pathogenic Escherichia coli and chemically synthesized to be complementary with the nucleotide sequence wherein the synthesized nucleotide has sequence shown by formula I, formula II or corresponding complementary sequence is used. An oligonucleotide having at least one of the sequences shown by formula I and formula II is functioned as a primer for chain reaction and a target nucleotide sequence is selectively amplified.