An assay method for the detection of Factor XIII in plasma is disclosed in which a primary amine derivative of biotin such as preferably 5-(biotinamido) pentylamine is incubated with a glutamine substrate and activated Factor XIII (Factor XIIIa) to form a biotinylated product which may be measured by convention detection assays. In a preferred embodiment, the biotinylated product is bound to a well in a microtiter plate or other solid support and the product is measured by a colorimetric assay which may be read by an automated spectrophotometric plate reader.
申请公布号
US5015588(A)
申请公布日期
1991.05.14
申请号
US19870114630
申请日期
1987.10.29
申请人
THE SAMUEL ROBERTS NOBLE FOUNDATION, INC.
发明人
LEE, KYUNG;PATTERSON, JR., MANFORD K.;BIRCKBICHLER, PAUL J.