摘要 |
<p>Production of gamma -linolenic acid, by growth of a diffused mycelial inoculum of Rhizopus arrhizus in a stirred, aerated nutrient medium containing a carbohydrate energy source and an inorganic nitrogen source at a carbon:nitrogen ratio of 20:1 to 60:1 maintained at a temperature of 25 DEG C.+/-2 DEG C. and a pH of 3.75 to 6.25 in the presence of a foam breaker, until a gamma -linolenic concentration of at least 1.0 g/liter of medium is reached, harvesting the organism, and extraction of lipid containing the gamma -linolenic acid.</p> |