发明名称 |
PRODUCTION OF PROTEINS USING HOMOLOGOUS RECOMBINATION |
摘要 |
Methods and compositions are provided for expression of mammalian genes in culture. An amplifiable gene is introduced by homologous recombination in juxtaposition to a target gene, the resulting combination of amplifiable gene and target gene transferred to a convenient host and the target gene amplified by means of the amplifiable gene. The resulting expression host may then be grown in culture with enhanced expression of the target gene.
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申请公布号 |
CA2045175(A1) |
申请公布日期 |
1991.05.07 |
申请号 |
CA19902045175 |
申请日期 |
1990.11.06 |
申请人 |
CELL GENESYS, INC. |
发明人 |
SKOULTCHI, ARTHUR I. |
分类号 |
C12N15/09;C07K14/505;C07K14/535;C07K14/59;C12N1/19;C12N5/10;C12N15/12;C12N15/81;C12N15/85;C12N15/86;C12N15/90;C12P21/00;C12Q1/68;C12R1/91;(IPC1-7):C12P21/00 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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