The manufacture and expression of large structural genes.

摘要

There is provided a method for the manufacture of linear, double stranded DNA sequences of a length in excess of about 200 base pairs and coding for expression of a predetermined continuous sequence of amino acids with a selected host microorganism transformed by a selected DNA vector including the sequence, a manufactured, linear, double stranded DNA sequence of a length in excess of about 200 base pairs and coding for the expression of a predetermined continuous sequence of amino acids by a selected host microorganism transformed with a selected DNA vector including the sequence, said sequence characterized by having at least one unique palindromic six base recognition site for restriction endonuclease cleavage at an intermediate position therein, a polypeptide product of the expression by an organism of such a manufactured DNA sequence, a biologically functional DNA microorganism transformation vector including such a manufactured DNA sequence and a reagent material comprising a radio labelled manufactured DNA sequence.