MONOCLONAL ANTIBODY AND HYBRIDOMA CAPABLE OF SECRETING SAME ANTIBODY

摘要

NEW MATERIAL:A monoclonal antibody inhibiting a biological activity of basic fibroblast growth factor(bFGF) and exhibiting no crossing-over reaction with bFGF inactivated by heating. USE:Useful for basic research of bFGF and diagnosis and treatment of malignant tumor by measuring bFGF which is malignant tumor maker in high precise. PREPARATION:A mouse is immunized with bFGF separated from cow brain, etc., and then a spleen cell of the above-mentioned mouse is fused with a mouse myeloma cell. Then cell capable of producing the aimed antibody from the resultant hybridoma is selected and the selected cell is proliferated in abdominal dropsy of mouse to provide the aimed antibody.