| 摘要 |
PURPOSE:To easily obtain a liposome useful for basic and application researches as a biomembrane model or a drug carrier for drug delivery, without using an organic solvent and a surfactant, by dispersing a liposome-membrane component substance in an aqueous solution and heating the mixture at a specific temperature. CONSTITUTION:A liposome dispersion is produced by dispersing 1 pt.wt. of a liposome-membrane component substance (e.g. phosphatidyl-choline in 3-100 pts.wt. of water, physiological saline water, buffer solution or an aqueous solution of sugar using an agitator. The dispersion is heated at or above the pretransition temperature(Tp) and below the main transition temperature(Tm) and held at the temperature for 5-20min. The treated dispersion is finally heated at >=Tm to obtain the objective liposome. The particle size can be uniformized by carrying out the above heat-treatment under irradiation with ultrasonic wave and the incorporation into the liposome membrane can be promoted by the use of 1 membrane component substance having high melting point in combination with the above substance. |
