| 摘要 |
PURPOSE:To preserve Basidiomycetes for a long period of time stably and safely by inoculating mycelia of Basidiomycetes on a wood powder-based medium for preservation containing sawdust sterilized with a polyhydric alcohol as an autifreezing agent, culturing and freezing at an ultralow temperature. CONSTITUTION:A wood powder-based medium for preservation containing at least sawdust is mixed with a polyhydric alcohol (e.g. glycerol or ethylene glycol) as an antifreezing agent and sterilized. Then mycelia of Basidiomycetes (e.g. Lentinus edodes) or a mold (e.g. koji mold) capable of growing in a wood powder-based medium are inoculated into the medium and cultured. Then the mycelia are multiplied to a given range, frozen at an ultralow temperature and stored. Consequently, the mycelia can be stored in a frozen state for a long period of time and the mycelia are optionally thawed, the thawed mycelia are partially used as an inoculation source for a medium for subculture or culture and the rest can be frozen again. |
