SIMULTANEOUS SEPARATION OF AMINO ACID AND AMINO SUGAR

摘要

PURPOSE:To achieve a higher speed by employing a buffer containing sodium citrate of below 0.08N as first eluate. CONSTITUTION:Eluates 1-3 and a regenerated liquid 4 are switched automatically with a valve 6 and sent to a column system with a pump 7. A sample is sent with an autosampler 10 to a separation column 11 of a solfonate type cation exchange resin. An eluate from a column 11 is mixed with a ninhydrine reaction liquid 5 being fed with a pump 8 by a mixer 12 and is heated while flowing through a reaction coil 13. Subsequently, it is detected as absorption of visible light with a detector 17 and calculated with a data processor 15. A density of sodium ion of the first eluate containing sodium citrate is made below 0.08N to allow separation into a neutral amino acid and amino acid being eluted with a second eluate. Thus, it is possible to separate amino acid from amino sugar with a single separation column thereby achieving a higher speed of a separating operation with simplification thereof.