| 摘要 |
A method of detecting and identifying amino acid derivatives produced by sequential cleavage of N-terminal residues from a polypeptide chain. The polypeptide is first reacted with an isothiocyanate to produce an N-terminal-thiocarbamyl polypeptide. This derivative is then cleaved to form a cyclic ATZ derivative, which is subsequently combined with a detectable alcohol in anhydrous acid to primarily produce a stable thiocarbamyl amino acid ester. This compound may be readily detected and identified.
|
