SUBSTRATES FOR HYDROLASES, A METHOD FOR THEIR PREPARATION AND THEIR USE

摘要

Chromogenic substrates of the formula A-O-B are described, in which A represents the radical in the ion A-O of a compound A-OH, whose absorbance is measured photometrically after hydrolysis of A-O-B, while 3 is the radical in a compound B-OH which makes the compound specific for the reaction with a given enzyme, and where A-OH is a hydroxynitropyridine, a hydroxynitrotenzopyranone, a dibenzox(or thi)azine or a dibenzopyranone or a derivative thereof, and B is a sugar or sugar derivative, an amino acid or an oligopeptide or an inorganic acid. These compounds can be used for the detection and determination of hydrolases.