| 摘要 |
PURPOSE:To obtain a vaccine, containing an antigenic protein, extracted and purified from pollens of HINOKI (Chamaecyparis obtusa Endl.) as an active ingredient and useful for diagnosing, preventing and treating pollinosis of SUGI (Cryptomeria japonica D. Don) and the HINOKI. CONSTITUTION:Pollens of HINOKI are extracted using a glucose solution containing phenol added thereto, sodium hydrogencarbonate solution, physiological saline solution, etc., preferably tris(hydroxymethyl)aminomethane (tris) hydrochloric acid solution as an extracting solvent according to a normally used method and the resultant crude extract is separated by a normally used method for purifying proteins to provide an antigenic protein having antigenicity common to the pollens of SUGI and HINOKI, 40000-45000 molecular weight and 6.5-6.8 isoelectric point measured by an electrophoretic method using polyacrylamide. A vaccine containing the aforementioned protein is subcutaneously or orally administered for a prescribed period to effectively inhibit production of IgE antibodies against pollinic antigen of the SUGI and HINOKI and simultaneously promote production of the IgG antibody and IgA antibody which are blocking antibodies against the pollinic antigen penetrated into mucous membranes or bodies. |
