发明名称 |
MATURE HETEROGENEOUS PROTEIN AND YEAST STRAIN IMPROVED PARTICULARLY FOR PRODUCTION OF HIRUDINE AND CORRESPONDING PRODUCTION METHOD FOR SAID HIRUDINE |
摘要 |
The invention relates to an improved system for the production of mature heterologous proteins, and in particular of hirudin, by yeasts, comprising an expression vector containing a sequence coding for the heterologous protein. This improvement is characterised by the amplification of the yeast KEX2 gene coding for the yscF endoprotease. The amplification is carried out either by integration of one or more copies of all or part of the KEX2 gene in the genome of the yeast, or by insertion of one or more copies of all or part of the KEX2 gene in the vector for expression of the heterologous protein. <IMAGE> |
申请公布号 |
JPH0372867(A) |
申请公布日期 |
1991.03.28 |
申请号 |
JP19900087339 |
申请日期 |
1990.03.31 |
申请人 |
TORANJIEENU SA |
发明人 |
IBU REMOWAANU;MARUTEIINU NUIEN;TEIRUMAN AAHASHIYUTETSUTAA |
分类号 |
C12N9/99;C07K14/815;C12N1/16;C12N1/19;C12N9/60;C12N15/09;C12N15/12;C12N15/15;C12N15/57;C12N15/81;C12P21/02;C12R1/865 |
主分类号 |
C12N9/99 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|