| 摘要 |
<p>Isomerically pure 5- and 6-succinimidylcarboxylates of rhodamine dyes are provided. A key feature in the preparation of the succinimidylcarboxylates is the use of stoichiometric amounts of di-N-succinimidylcarbonate and 4-dimethylaminopyridine for converting the 5- and/or 6-carboxyrhodamines to N-hydroxysuccinimide esters. In chain termination DNA sequencing procedures, using the pure isomeric forms of the activated rhodamines prevents generation of spurious sequence data because of the different electrophoretic mobilities of the isomers.</p> |
