摘要 |
PURPOSE:To efficiently obtain a pharmacodynamic component from Eucommia ulmoldes by culturing a plant tissue piece of Eucommia ulmoldes. CONSTITUTION:A plant tissue piece collected from stem part or upper hypocotyl is inoculated into B-5 (Gamborg) 1966 or WPM(Woody Plant medium) 1982 culture medium to which auxicin and cytokinin were added and subjected to an induction culture of callus at 25+ or -2 deg.C and for 10-24hr under a light irradiation of 100-200lux to provide the plant tissue-cultured callus of Eucommia ulmoldes containing an effective component for medical use of pinoresinol di-o-beta-D- glucoside, Aucubin, syringin, liriodendrin, etc., having antihypertensive action. |