ANTI-HUMAN BCDF MONOCLONAL ANTIBODY AND DETERMINATION OF HUMAN BCDF USING THE SAME

摘要

PURPOSE:To produce an anti-human BCDF monoclonal antibody by preparing and culturing a hybridoma of e.g. HH61-8 cell capable of producing a specific monoclonal antibody against human BCDF. CONSTITUTION:A hybridoma is synthesized by fusing a myeloma cell to an antibody-producing cell. The hybridoma is preferably a hybridoma of a mouse myeloma cell and a mouse lymph node cell or spleen cell immunized with human BCDF. Concrete preferable examples of the hybridoma are HH61-8 cell (FERM P-10874) and HH61-10 cell (FERM P-10875). The hybridoma is transplanted and proliferated in the peritoneal cavity of a histocompatible animal, etc., and the anti-human BCDF monoclonal antibody produced in the ascites of the animal is recovered and purified.