| 摘要 |
PURPOSE:To detect swine epidemic pneumonia mycoplasma by using a DNA having nucleotide base sequence of a specific structural formula as a probe and performing DNA-hybridization. CONSTITUTION:A DNA having nucleotide base sequence selected from group expressed by formula I, formula II, formula III, formula IV or formula V is used as a probe and swine epidemic pneumonia mycoplasma (Mycoplasma hypopneumoniae) is detected by adopting a DNA-hybridization method. Said detecting method of swine epidemic pneumonia mycoplasma using DNA as a probe is able to be performed in a shorter time (about 2 day) in comparison with normal separation detecting method. Furthermore, said method is able to be adopted about the sample of e.g. pituita of infected swine having high viscosity by treating the sample with dithiothreitol, etc. |
