Lipase, its production and use for assay of triglycerides

摘要

Lipase isolated from Penicillium characterized by the following properties: (1) Efficiently hydrolyzes triglycerides of fatty acids having 4-18 carbon atoms; (2) Hydrolysis of such triglycerides with such lipase produces at least 5 moles of glycerol per 100 moles of fatty acid; (3) Optimal pH range for said hydrolysis 5-7; (4) Stable in the pH range of 4.5-6; (5) Optimal temperature range for activity 35 DEG -40 DEG C.; (6) thermal stability up to about 35 DEG C.; (7) Activated by surface-active agents, but not substantially inhibited by such surface-active agents in concentration up to 5%; (8) Molecular weight about 1000,000 to 120,000; (9) Isoelectric point about pH 3.84.