| 摘要 |
The present invention involves a method for separating biological molecules by subjection of said molecules to a separation system in a gel medium. The method most particularly involves the use of a gel slab suitable for such separations which is readily equilibrated with an appropriate solvent for the chosen separation system. An important aspect of the present invention involves the initial preparation of a gel slab comprising between about 0.5% and about 2.0% agarose and between about 1% and about 3.0% linear water-soluble and substantially nonionic polyacrylamide. A preferred gel slab of the present invention contains between about 1% and 3% agarose and about 3% linear, water-soluble, substantially nonionic polyacrylamide. The gel slabs of the present invention are preferably between about 1 mm and 0.5 mm in thickness. Said aged gel slab is then dried to produce a gel precursor sheet. A preferred drying procedure is to subject the gel slab to a stream of warm air to maintain a preferable gel surface temperature of about 45 DEG -50 DEG C., a 0.5 mm gel slab taking about 6-8 min to dry under these conditions. The dried gel precursor sheet, preferably maintained in a dried state for less than about 24 hr, is immersed for at least about 30 min, preferably at about 20 DEG C., in an aqueous separation solvent compatible with the separation system to be utilized. Aqueous separation solvents usable for gel rehydration include those comprising one or more of detergents (preferably nonionic), urea (up to about 10M), ampholytes and pH buffers, for example, depending upon the particular separation system to be used for separation of the biological molecules. Usable separation systems include electrophoresis, immunodiffusion and isoelectric focusing, for example.
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