MONOCLONAL ANTIBODY RESISTANT AGAINST HUMAN CD4 PEPTIDE

摘要

NEW MATERIAL:A monoclonal antibody reactive with a human CD4 peptide (70-132). USE:A reagent for analyzing the infection mechanism of HIV which is a source virus of AIDS. PREPARATION:For example, a human CD4 peptide (70-132) is administered together with a Freund's complete adjust into the abdominal cavity of a BALB/ C mouse to immunize the mouse, followed by additionally immunizing the mouse and finally immunizing the mouse. Splenic cells are collected from the immunized mouse, fused with mouse myeloma cells and cultured in a HAT medium to prepare hybridomas. The hybridomas are cultured and a strain having an ability to produce an antibody specific to the CD4 peptide is selected from the supernatant of the cultured product. The strain is cloned by a limiting dilution analysis, etc., and the monocloned hybridoma is cultured to provide the monoclonal antibody.