发明名称 |
PROCESS FOR PREPARING A PROTEIN BY A FUNGUS TRANSFORMED BY MULTICOPY INTEGRATION OF AN EXPRESSION VECTOR |
摘要 |
A process is disclosed for preparing a protein by a eukaryote transformed by multicopy integration of an expression vector into the genome of a yeast, such as Saccharomyces, Hansenula and Kluyveromyces, or of a mould such as Aspergillus, Rhizopus and Trichoderma, said expression vector containing both an "expressible gene" encoding said protein and a so-called "deficient selection marker needed for the growth of the yeast or mould in a specific medium", such as the LEU2d, TRP1d or URA3d gene, in combination with a ribosomal DNA sequence, resulting in stable high copy integration of 100-300 copies per cell. This multicopy integration results in a increased production of the desired protein, which can be guar a-galactosidase, an oxidase or a hydrolytic enzyme such as a lipase. |
申请公布号 |
WO9100920(A3) |
申请公布日期 |
1991.03.07 |
申请号 |
WO1990EP01138 |
申请日期 |
1990.07.09 |
申请人 |
UNILEVER PLC;UNILEVER N.V. |
发明人 |
GIUSEPPIN, MARCO, LUIGI, FREDERICO;LOPES, MARIA, TERESA, SANTOS;PLANTA, ROELF, JOHANNES;VERBAKEL, JOHANNES, MARIA, ANTONIUS;VERRIPS, CORNELIS, THEODORUS |
分类号 |
C12N15/09;C12N1/15;C12N9/20;C12N9/40;C12N15/81;C12P21/02;C12R1/645 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|