发明名称 |
ENHANCED NUCLEIC ACID AMPLIFICATION PROCESS |
摘要 |
This invention relates to an improved process for amplifying a specific nucleic acid sequence. The process involves synthesizing single-stranded RNA, single-stranded DNA and double-stranded DNA. The single-stranded RNA is a first template for a first primer, the single-stranded DNA is a second template for a second primer, and the double stranded DNA is a third template for synthesis of a plurality of copies of the first template. A sequence of the first primer or the second primer is complementary to a sequence of the specific nucleic acid and a sequence of the first primer or the second primer is homologous to a sequence of the specific nucleic acid. The amplification process may be used to increase the quantity of a specific nucleic acid sequence to allow detection, or to increase the purity of a specific nucleic acid sequence as a substitute for conventional cloning methodology. |
申请公布号 |
WO9102818(A1) |
申请公布日期 |
1991.03.07 |
申请号 |
WO1990US04733 |
申请日期 |
1990.08.23 |
申请人 |
CANGENE CORPORATION |
发明人 |
MALEK, LAWRENCE, T.;DAVEY, CHERYL;HENDERSON, GRAHAM;SOOKNANAN, ROY |
分类号 |
C12N15/09;C12N15/00;C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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