CHROMOSOMAL DNA SEQUENCE, EXPRESSION VECTOR FOR HUMAN TISSUE PLASMINOGEN ACTIVATING FACTOR, CULTURED CELLS TRANSFECTED WITH SAME AND METHOD OF PRODUCING SAID ACTIVATING FACTOR
摘要
<p>The invention provides a chromosomal DNA sequence free of the SmaI recognition site between the 8th and the 9th exon; an expression vector which has a DNA sequence comprising a chromosomal DNA sequence coding for human tissue plasminogen activating factor and a promoter region functioning in cultured cells derived from a gene other than human tissue plasminogen activating factor as joined to said chromosomal DNA sequence; cultured cells transfected with such expression vector for human tissue plasminogen activating factor; and a method of producing human tissue plasminogen activating factor which comprises cultivating cultured cells transfected with such expression vector for human tissue plasminogen activating factor and isolating the human tissue plasminogen activating factor produced by the cells.</p>
