摘要 |
PURPOSE:To enable to efficiently, inexpensively and massively prepare dihomo-gamma- linolenic acid by culturing a microorganism having a dihomo-gamma-linolenic acid- producing activity in a medium containing curcumin. CONSTITUTION:A solvent solution such as ethanol containing curcumin in an amount of 0.01-10g per l of the medium is compounded with a carbon source such as gamma-linolenic acid-containing oil, a nitrogen source such as a yeast extract, an inorganic salt such as KH2PO4, etc., to prepare a liquid medium. A bacterium such as Coridiobolus nanodes CBS 183/62 strain is inoculated in the prepared medium and subsequently cultured at 10-40 deg.C for 3-10 days with aerating and stirring. The cultured product is centrifuged and the separated cells are subjected to an extraction treatment and a subsequent purification treatment to prepare dihomo-C-linolenic acid. An agent for inhibiting the unsaturation reaction at the 5-position of a fatty acid in the fats or oils of microorganisms or animal cells contains curcumin in an amount of 0.01-100 mg/g of the dry cells as a main ingredient. |