The present invention concerns a method to catalyze reporter deposition to improve detection or quantitation of an analyte in a sample by amplifying the detector signal which comprises immobilizing an analyte dependent enzyme activation system which catalyzes deposition of reporter by activating a conjugate consisting of a detectably labeled substrate specific for the enzyme system, said conjugate reacts with the analyte dependent enzyme activation system to form an activated conjugate which deposits substantially wherever receptor for the activated conjugate is immobilized, said receptor not being reactive with the analyte dependent enzyme activation system. In another embodiment the invention concerns an assay for detecting or quantitating the presence or absence of an analyte in a sample using catalyzed reporter deposition to amplify the reporter signal. Also described are novel compounds which can be used as reagents to prepare HABA-type conjugates.
申请公布号
WO9011523(A3)
申请公布日期
1991.02.21
申请号
WO1990US01569
申请日期
1990.03.28
申请人
E.I. DU PONT DE NEMOURS AND COMPANY
发明人
BOBROW, MARK, NORMAN;EBERSOLE, RICHARD, CALVIN;LITT, GERALD, JOSEPH;MORAN, JOHN, RICHARD