| 摘要 |
<p>A plant thin-cell-layer (TCL) culture system has been developed to study root, vegetative shoot, and flower morphogenesis. The effects of hormone concentrations, pH, and light quality and quantity on TCL morphogenesis were analyzed. Roots, vegetative shoots, and flowers formed on TCLs cultured on various liquid media that had been adjusted to the same pH. Evidence is presented showing that the effects of light on TCL morphogenesis are associated with light-mediated degradation of indol-3-butyric acid (IBA) in the medium. Evaluation of the organogenesis that occurred in TCLs cultured on a range of IBA and kinetin concentrations revealed that the quantity and type of organs formed were dependent upon the concentration of IBA and kinetin in the medium. The TCL assay system is useful for studies of the molecular changes associated with organogenesis and for the screening, identification, and purification compounds capable of affecting plant morphogenesis.</p> |
