发明名称 |
PROCESS FOR THE SITE-DIRECTED INTEGRATION OF DNA INTO THE GENOME OF PLANTS |
摘要 |
The present invention provides a method for site-directed integration of DNA-sequences into the genome of plants via homologous recombination, by transforming said plants using the DNA-transfer system of Agrobacterium, in which the transforming DNA comprises in its most simple form a region homologous to the target locus, as well as a region which is different from the target locus either next to one or between two T-DNA borders. Special constructs are provided, which in its most complete form have the general structure (I), in which box (1) and (7) represent T-DNA borders, boxes (2) and (6) comprise functional expression cassettes containing negative selection genes, box (3) provides a region of homology with the target locus promoting recombination, box (4) represents a DNA sequence containing a mutation with respect to the target locus, box (5) represents a functional expression cassette containing a positive selection gene, and box (E) comprises a DNA sequence which is homologous to a region adjacent of the target locus, or in the vicinity of the target locus, which promotes homologous recombination. |
申请公布号 |
WO9102070(A1) |
申请公布日期 |
1991.02.21 |
申请号 |
WO1990NL00106 |
申请日期 |
1990.07.26 |
申请人 |
MOGEN INTERNATIONAL N.V.;RIJKSUNIVERSITEIT LEIDEN |
发明人 |
OFFRINGA, REMKO;DE GROOT, MARCELLUS, JOHANNES, AUGUSTINUS;HOOYKAAS, PAUL, JAN, JACOB;VAN DEN ELZEN, PETRUS, JOSEPHUS, MARIA |
分类号 |
A01H1/00;A01H5/00;C12N1/21;C12N5/10;C12N9/12;C12N9/88;C12N15/09;C12N15/82 |
主分类号 |
A01H1/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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