| 摘要 |
PURPOSE:To make it possible to obtain sorbic acid in good yield and high purity by treating sorbic aldehyde with one or more kind of microorganisms selected from the genus Agrobacterium, etc. CONSTITUTION:One or more kind of microorganisms selected from among the genera Agrobacterium, Azotobacterium, Erwinia (e.g. Erwinia carotovora) and Staphylococcus are cultured in a culture medium containing glucose, urea, etc., to provide the cultured product. A bacterial cell obtained by collecting from the cultured product is suspended at an amount of 0.2-20g (as a dried bacterial cell) per 1 of a physiological saline, etc., to provide a suspension. Then 1-10wt.% sorbic aldehyde is added to the above-mentioned suspension to dissolve sorbic aldehyde and then air or O2, etc., is blown into the suspension so as to keep O2 concentration to >=1ppm and sorbic aldehyde is reacted in the suspension at 10-70 deg.C and pH 3-10 for 0.1-200hr while stirring. Then the resultant reaction product is separated by centrifugal separation method and purified to provide sorbic acid. |
