发明名称 |
OPTIMIZED FERMENTATION PROCESSES FOR THE PREPARATION OF FOREIGN PROTEINS IN E. COLI |
摘要 |
The invention describes optimised fermentation processes for the production of foreign proteins in E.coli using the lac promoter or improved lac promoter (e.g. tac, trc). After the initial growth phase with glucose as carbon source, product formation is induced (1) by IPTG with glucose limitation or (2) by lactose with lactose limitation or (3) by IPTG and lactose with lactose limitation. The limitation of glucose or lactose is such that the partial pressure of oxygen remains above 10%. |
申请公布号 |
AU6000590(A) |
申请公布日期 |
1991.02.07 |
申请号 |
AU19900060005 |
申请日期 |
1990.08.01 |
申请人 |
BEHRINGWERKE AKTIENGESELLSCHAFT |
发明人 |
MATHIAS GROTE |
分类号 |
C12P21/00;C07K14/47;C12N1/20;C12N15/09;C12N15/12;C12N15/72;C12P21/02;C12R1/19 |
主分类号 |
C12P21/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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