PRODUCTION OF NITRATE REDUCTASE

摘要

A novel thermostable formate linked nitrate reductase enzyme system is produced from a novel strain of the genus Thermus. The enzyme system is suitable for determination of nitrate down to the concentration of 0,005 ppm NO3-N. The reduction of nitrate is suitable for this system in boiling water. The reduction of nitrate to nitrite takes 10 minutes to complete in boiling water and is not inhibited by oxygen. For this reduction a whole cell preparation of Thermus anaerobicus, or a partial purification thereof, is used. A conventional color indicator system is used to quantify nitrite.