| 摘要 |
<p>PURPOSE:To enable mass production of patchouli by culturing protoplasts of patchoui in a culture medium for protoplast cultivation having a specific ammonia nitrogen concentration. CONSTITUTION:Patchouli protoplasts are inoculated in a medium consisting of 200 to 800mu protoplast culture medium of less than 2mM ammonia nitrogen concentration containing 2 to 4mg/l of 2,4-D, 0.5 to 2.0mg/l of kinetin, 2 to 4mg/l of NAA, 2 to 6wt.% of sucrose and 0.4 to 0.6M of mannitol and, when needed, 400 to 1,500mul of solid medium and clutured at 25 to 27 deg.C under 200 to 700lux without addition of fresh medium to form colonies. Then, the colonies are inoculated in the basal medium containing less than 10mg/l of auxin and 5mg/l of cytokinin or only cytokinin and cultured at 25 to 27 deg.C under light for 1 to 2 months until they differentiate into plant bodies.</p> |
