Gene encoding M-CSF and related recombinant systems thereof.

摘要

Novel genes coding for human M-CSF are obtained. The unique genes are first derived from human monocytic cells, and then modified using gene manipulation techniques. Each gene construct is inserted into an expression vector which is used to transform an appropriate host. The transformed host cells produce efficiently unique recombinant human M-CSF, including urinary M-CSF, which is purified. High productivities are achieved in both animal cells and E. coli via the genes.