Determination and detection of antibody and its immunoglobulin class.

摘要

This method can determinate and detect an antibody in a biological fluid sample against a specific antigen and its immunoglobrin class. This method comprises the step of: -----providing a first reagent of insoluble substrate particles carrying the antigen on the surface thereof and a second reagent of insoluble substrate particles carrying on the surface thereof a substance paticulary reactive to a specific immunogloblin class, the latter reagent containing a magnetic material; -----reacting the sample with both of the reagents in a reaction system; -----agglomerating the antigen-carrying particles together with the magnetic material-containing particles through the antibody; -----separating both of the unreacted magnetic material-containg particles and the resultant agglomerate by applying a magnetic field to the reaction system; and -----visually or spectrophotometrically estimating an amount of the antigen-carrying particles which remains unreacted in the reaction system.