Method for detecting hydrogen peroxide employing triaryl- and trihetarylmethane derivatives as redox indicators

摘要

A method for detecting hydrogen peroxide wherein a sample is contacted with a peroxidase or a peroxidatively-active substance and a redox indicator of the following in which A and D independently of one another represent phenyl, pyridyl or imidazolyl, G represents O, CH2 or S, m represents the number zero or one, and X represents O, or -NR1-NR2- R1 and R2, independently of one another, denote hydrogen, alkyl, cycloalkyl, aryl or aralkyl, or -NR1R2 together represent a pyrrolidine, pyrazoline, piperidine, piperazine or morpholine radical and T denotes hydrogen, hydroxyl, alkyl, aryl, alkoxy, phenoxy, SO3H, -COOH or whereby a color change is brought out if hydrogen peroxide is present.