| 摘要 |
2063986 9102073 PCTABS00003 An immunoassay apparatus is disclosed for the quantitative determination of the concentration of a target ligand in a liquid sample. The apparatus contains top (10) and middle (14) plates having holes (12, 18). The holes in the two plates are in axial alignment and the holes in the middle plate have sidewalls (22) that extend below the bottom surface of the middle plate. A liquid permeable membrane (16) is placed between the plates. The apparatus contains a bottom chamber (40) open to the bottom surface of said middle plate, a vacuum port (42) and can hold a microtiter plate containing a plurality of wells (52). Liquid is placed in the holes in the top plate and vacuum is created in the chamber. The liquid is drawn at a controlled rate directly through the membrane without lateral dispersion, through the holes in the middle plate and, into the bottom chamber.
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