摘要 |
<p>Nucleotides can be linked from a 5'-phosphate residue to a support via a dithio (-S-S-) linkage. Polynucleotides thus immobilised can be used in the polymerase chain reaction (PCR) at which a temperature of 90°C is typically required. The -S-S- linkage can be produced by reaction of a thiolated support with a 5'-(thiol-terminated) polynucleotide. For ease of hybridisation of the immobilised polynucleotide, the sequence to be hybridised should be adequately spaced from the support. The invention is useful in any situation in which a polynucleotide such as DNA is required to be separated as a solid phase from a liquid phase, especially in heterogeneous assays and to isolate products of the PCR.</p> |