Analytical element and method for the determination of creatinine.

摘要

A multilayer analytical element comprises a support having thereon in the following order: a) a first reagent layer comprising sarcosine oxidase, a rate limiting amount of creatinine amidohydrolase; a peroxidative substance and imidazole leuco dye which is capable of providing a detectable dye in the presence of said peroxidative substance and hydrogen peroxide; b) a second reagent layer containing creatinine amidinohydrolase; and c) a porous spreading layer; characterized in that a high NEG oxidase activity sarcosine oxidase having an activity of at least 300 I.U./m<2> relative to N-ethyl glycine is present in i) the porous spreading layer; ii) a separate layer between the spreading layer and the layer containing creatine amidinohydrolase; iii) the layer containing creatine amidinohydrolase or iv) all of such layers i) - iii). p