| 摘要 |
PURPOSE:To provide the artificial blood vessel which is used for rebuilding of the blood circulation in a small-diameter blood vessel and vein blood vessel by treating the wall of the blood vessel of a mammal with org. acid or guanidine hydrochloride, then culturing the smooth muscle cells and endothelio cells. CONSTITUTION:The blood vessel treated with the org. acid, such as formic acid, or the guanidine hydrochloride rebuilds the blood vessel wall. The smooth muscle cells and endothelio cells are cultured for this purpose. The rabbit smooth muscle cells, swine smooth muscle cells, bovine smooth muscle cells, canine smooth muscle cells, etc., are sued for the smooth muscle cells. The swine endothelio cells, canine endothelio cells, etc., are used for the endothelio cells. The culture is executed by a method of washing the blood vessel wall, which is treated with the sterilized formic acid, etc., with distilled water and physiological salt soln. and is permeated in a Dulbecco disintegration Eagle medium and is then cultured in the suspension of the swine smooth muscle cells or after the suspension (c) of the swine smooth muscle cells is injected at about 0.2ml/graft by an injection needle of 27G to the vessel wall, the cells are cultured. The concn. of the aq. formic acid to be used is 70 to 95%, more preferably 80 to 90% and the treatment time is one day to one week. The treating temp. is <=5 deg.C, more preferably <=4 deg.C. |
