PROCESS FOR PREPARING AND PURIFYING AN ANTICOAGULANT

摘要

Process for Purifying Annexines The process comprises the steps of a) adjusting the pH value of homogenised cellular material to 8.0 to 10.0; b) adding at least one bivalent cation selected from Ca2+, Cd2+, Zn2+, Mn2+ and Co2+; c) adding a phospholipid; d) washing insoluble cell residue to remove soluble constituents; and e) extracting annexines from the cell residue using chelating agents. Overall yields of annexines are raised to more than 80%.