发明名称 |
TARGETED LIPOSOMES AND METHODS FOR LIPOSOME-PROTEIN COUPLING |
摘要 |
This invention relates to a method for synthesizing a substantially pure reactive lipid including, for example, N-[4-(p-maleimidophenyl)-butyryl]phosphatidylethanolamine (MPB-PE) and related compositions. The compositions of the present invention are useful as coupling agents and may be incorporated into liposomes and subseuquently coupled to proteins, cofactors and a number of other molecules. A preferred coupling method is also disclosed as are protein conjugates. The present invention also relates to a general method for producing sized protein-liposome conjugates exhibiting enhanced blood circulation times. The present invention also relates to the sized protein-liposome conjugate compositions produced by the method of the present invention. The protein-liposome conjugates of the present invention preferably range in size from about 75 nm to about 200 nm. The liposomes of the invention may have a trans-membrane potential across their membranes, and may be dehydrated. In addition, the composition may contain ionizable bioactive agents such as antineoplastic agents, and may be used in diagnostic assays. |
申请公布号 |
WO9100289(A2) |
申请公布日期 |
1991.01.10 |
申请号 |
WO1990US03582 |
申请日期 |
1990.06.22 |
申请人 |
THE LIPOSOME COMPANY, INC. |
发明人 |
LOUGHREY, HELEN, C.;CULLIS, PIETER, R.;BALLY, MARCEL, B.;CHOI, LEWIS, S., L.;WONG, KIM, F. |
分类号 |
A61K9/127;A61K31/70;A61K38/00;A61K39/395;A61K47/14;A61K47/24;A61K47/42;A61K47/48;A61K49/00;A61P35/00;B01J13/00;C07D207/40;C07D207/404;C07D207/46;C07F9/10;C07K1/113;C07K14/00;C07K16/00;G01N33/53;G01N33/544;G01N33/577 |
主分类号 |
A61K9/127 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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