EXPRESSION VECTOR AND METHOD FOR CONTROLLABLE PRODUCTION OF PROTEINS IN EUKARYOTES

摘要

An expression vector containing a controllable promoter inserted into a eukaryotic DNA contains as promoter the truncated DNA sequence of the alpha -glucosidase pI promoter region in which not less than the entire region upstream of nucleotide -867 and not more than the entire region upstream of nucleotide -140 is deleted. For the controllable production of homologous and heterologous proteins in eukaryotic host cells, a DNA sequence coding for the required protein is inserted into an expression vector of this type, the resulting vector is inserted by transformation into a suitable host cell, the cells are cultivated and the produced protein is isolated from the cells or their nutrient medium.