发明名称 |
High gel strength low electroendosmosis agarose |
摘要 |
Purified agarose suitable for rapid electrophoresis, characterized by a sulfate content of less than 0.2 wt % but greater than zero, a pyruvate content of 0-0.1 wt %, and a nitrogen content of 0-0.02 wt %. Gels prepared from the agarose exhibit a gel strength at 1.0 wt % concentration of at least 1200 g/cm2, substantial absence of DNA binding in 0.07 M or less tris acetate buffer, and an electroendosmosis (EEO) at 1.0 wt % concentration of 0.05 or less. Agaroses are purified to provide the low EEO material by dissolving agarose or alkali-modified agar in an aqueous medium buffered at a pH of 6.0 to 8.0 and containing no more than 2.0 nM salt as chloride, and precipitating the agarose by contact with a lower alkanol.
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申请公布号 |
US4983268(A) |
申请公布日期 |
1991.01.08 |
申请号 |
US19890389141 |
申请日期 |
1989.08.03 |
申请人 |
FMC CORPORATION |
发明人 |
KIRKPATRICK, FRANCIS H.;GUISELEY, KENNETH;PROVONCHEE, RICHARD;NOCHUMSON, SAMUEL |
分类号 |
B01D57/02;B01J13/00;C07K1/24;C07K1/26;C08B37/00;C08B37/12;C12M1/00;G01N27/447 |
主分类号 |
B01D57/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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