MIXTURE OF AMINO ACID DERIVATIVES, PROCESS OF PRODUCING THE MIXTURE AND USE OF THE MIXTURE FOR QUANTITATIVE DETERMINATION OF THE AMINO ACIDS

摘要

A new mixture of fluorescent amino acid derivatives suitable for use in quantitative amino acid analysis is provided in which all primary amino acids are present in one kind of derivatization whereas all secondary amino acids are present in another kind of derivatization. …The process for recovering the mixture comprises the steps of derivatization of the primary amino acids of an amino acid mixture with ortho-phthalaldehyde (OPA) and of derivatization of the secondary amino acids with fluorenylmethylchloroformate (FMOC) in the presence of acetonitrile. The mixture can be obtained automatically and is highly suitable for fast analysis by reversed-phase liquid chromatography.