EPITHELIAL CELL LINE EXPRESSING A CYSTIC FIBROSIS PHENOTYPE

摘要

An airway epithelial cell line (CF/T43) was developed by infecting cultured cystic fibrosis (CF) airway epithelial cells with the pZIPneoSV(X)1/SV40T retrovirus and selecting for genetian (G418) resistance and ion transport properties. The distinctive chloride secretory phenotype of CF/T43 [an apical membrane chloride permeability (Pcl-) activated by calcium-mediated, but not by adenosine 3',5'-monophosphate (cAMP)-dependent agonists] was not perturbed by SV40T-induced cell transformation. Epithelial cell lines generated from CF cells with the SV40T gene can be used to test candidate CF genes, to evaluate the molecular mechanisms responsible for the CF phenotype and to test putative therapeutic CF drugs.