| 摘要 |
An ion exchange method for the separation of hemoglobin A2 from other hemoglobin components normally present in human blood involves the use of three buffer solutions in succession, with specified ionic strengths and pHs. The first has a phosphate buffer at 1 to 20 mM and a pH of 6.5 to 6.9; the second has phosphate buffer at 15 to 55 mM and a pH of 6.4 to 6.8; and the third has phosphate buffer at 60 to 100 mM and a pH of about 6.4 to 6.8. The first is continued until substantially all hemoglobin A1a and A1b has eluted from the column, the second until substantially all A1c, F, and A0 is eluted, and the third for the elution of A2.
|
