| 摘要 |
<p>PURPOSE:To obtain the subject adventive root useful as a medicine in high yield by culturing the adventive root in specific concentrations of nitric acid ion and sugar so as to be capable of culturing the adventive root at a high concentration when adventive root of tropane-based alkaloid producing plant is subjected to liquid culture. CONSTITUTION:A liquid culture medium or culture ingredient is fed to a liquid medium obtained by adding an adventive root of tropane-based alkaloid producing plant (e.g. Duboisia myoporoides) to a liquid medium, preferably of Nitsch & Nitsch, etc., containing a plant hormone, etc., so as to retain 10 to 30mM nitric acid and 2 to 10% sugar concentrations during culturing period and the adventive root is cultured at a high concentration (concentration of tissue-culture material at culture starting time of >=25g/l by fresh weight or concentration of the material at culture finishing time of >=200g/l in a culture tank) to provide the objective adventive root.</p> |
