GENE CODING PRECURSOR OF CHLOROPHYLL-LOCALIZED GLUTAMINE SYNTHETASE

摘要

NEW MATERIAL:The gene having an amino acid sequence of formula I (A is alanine; R is arginine; N is asparagine; D is aspartic acid; C is cysteine; Q is glutamine; E is glutamic acid; G is glycine; H is histidine; I is isoleucine; L is leucine: K is lysine; M is methionine; F is phenylalanine; P is proline; S is serine; T is threonine; W is tryptophan; Y is tyrosine; V is valine) and coding a rice chlorophyll localized glutamine synthetase precursor containing a signal peptide. USE:Production of vegetables resistant to herbicide. PREPARATION:cDNA of a cDNA library obtained from rice leaf of about 30 days after transplantation is hybridized with a probe of RGSC (partial cDNA) described in Japanese Patent Application Sho 63-225816. A fragment having long cDNA chain length is selected from the above hybrids and introduced into a plasmid. The obtained transformant is cultured to amplify the integrated cDNA.