PURIFICATION OF L-ASPARAGINASE

摘要

The present disclosure is directed to a process for purifying an enzyme preparation of L-asparaginase obtained from an L-asparaginase-producing microorganism belonging to the genus Serratia, wherein said microorganism produces together with the L-asparaginase, factors which inactivate the enzymatic activity of L-asparaginase; the improvement which comprises separating the inactivating factors from L-asparaginase during some stage of the purification process by salting out said factors with the addition of an inorganic salt and recovering purified L-asparaginase having an effective anti-tumor activity.