摘要 |
<p>A coincidence arbitration system (Figs. 3 and 4) for a sorting cytometer (10) which detects and sorts particles (60, 62 and 64) using a flow chamber (12) and a stream of droplets (58). Those droplets (58) selectively containing particles (60, 62 and 64) are sorted by selectively applying a charge (SORT) thereto and passing the droplets (58) through deflection plates (54 and 56). After detection, the particle data is delayed and analyzed to make sort decisions. The delay is for a period of one and one fourth droplet time periods less than the time the particle (60, 62 and 64) is formed into a droplet (58). At the time the command (SORT) is issued creating the charge on the droplet (58) to be formed. The coincidence arbitration circuit (Figs. 3 and 4) looks four quarter droplet times ahead and behind of the event to determine whether a coincidence is occurring, and if so, whether the coincidence is with a similar type of particle (60, 62 and 64) or a different type of particle (60, 62 and 64). Based on this information, appropriated sort or no-sort commands (SORT) are issued.</p> |