Process for the separation of macromolecules.

摘要

A process for the electrophoretic separation of charged macromolecules which comprises applying to said macromolecules a periodic sequence of pulses comprising, in each period, a multiplicity of electric field pulses of negative and positive polarities wherein the negative polarity pulses are applied for a longer total time duration (t2) than the positive polarity pulses, and the average intensity (V2) of the negative polarity pulses is less than the average intensity (V1) of the positive polarity pulses.